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rabbit α-s100b (Agilent technologies)

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Agilent technologies rabbit α-s100b
Rabbit α S100b, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+%CE%B1-s100b/pmc08654303__sciadv__abj1598_sm-148-46-49
Average 90 stars, based on 1 article reviews

rabbit α-s100b - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch
Article Snippet: The following primary antibodies were used in this study: rabbit α-Calbindin (1:1,000; Swant), rabbit α-Calretinin (1:1,000; Swant), sheep α-CGRP (1:1,000; Abcam), rabbit α-cMaf (1:5000; C Birchmeier, MDC, Berlin), goat α-cRet (1:250; R&D Systems), goat α-CTB (1:4000; List Laboratories), rabbit α-DsRed (1:1000; Clontech), mouse α-Gephyrin (1:8000; Synaptic systems); rabbit α-GFAP (1:500; Dako), chicken α-GFP (1:000; Aves), goat α-GFP (1:1000; Abcam), rabbit α-GRPR (1:1000; Abcam), rabbit α-GRPR (1:100; MBL), guinea pig α-Lmx1b (1:1000; M Goulding), mouse α-NeuN (1:1000; Millipore), rabbit α-NK1R (1:500; Advanced Targeting Systems), rabbit α-NPY (1:1000; Peninsula Lab), rabbit α-Parvalbumin (1:1000; Swant), rabbit α-Pax2 (1:200; Zymed), rat α-RFP (1:1000; Chromotek), rabbit α-PKCg (1:1000; Santa Cruz), goat α-RORa (1:100; Santa Cruz), rabbit α-S100b (1:500; Dako), goat TrkB (1:1000; R&D Systems), goat α-TrkC (1:1000; R&D Systems), guinea pig α-VGAT (1:1000; Synaptic Systems), guinea pig α-vGluT1 (1:1000; Millipore).

Article Title: Injury-induced ASCL1 expression orchestrates a transitory cell state required for repair of the neonatal cerebellum
Article Snippet: List of antibodies Target Catalog Number Company Dilution goat α-SOX2 AF2018 R&D System 1/200 (adult)1/500 (pups) rabbit α-PAX2 71600 Invitrogen 1/500 rabbit α-PAX6 AB2237 Millipore 1/500 rat α-GFP (CFP) 04404-84 Nacalai Tesque 1/1000 mouse α-ASCL1 556604 BD Pharmingen 1/1000 guinea pig α-PVALB 195-004 Synaptic Systems 1/1000 rabbit α-S100B Z0311 DAKO 1/1000 mouse α-NeuN MAB3777 Millipore 1/1000 rabbit α-HOPX HPA030180 Sigma (Prestige Ab) 1/1000 % cells in TdT+ cells at P30 Tm at P0 (n=3) Tm at P5 (n=3) Cell type nonIR (n=4) IR at P1 (n=3) nonIR (n=5) IR at P1 (n=4) ML Interneurons 95.41 ± 2.36 88.58 ± 0.79 96.28 ± 4.75 81.23 ± 4.57 Granule cells 0.69 ±0.48 0.69 ± 0.41 1.0 ± 1.4 11.83 ± 2.00 Bergmann glia 0.26 ± 0.13 0.04 ± 0.03 0 ± 0 0.35 ± 0.29 IGL Astrocyte 1.42 ± 0.83 5.75 ± 0.76 0.95 ± 1.60 1.06 ± 0.61 WM Astrocyte 2.21 ± 1.06 4.94 ± 1.51 1.04 ± 1.55 5.52 ± 1.86 Table S11.

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Primer sequences and general conditions used to perform real-time qPCR

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Primer sequences and general conditions used to perform real-time qPCR

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques:

Experimental conditions used to perform western blot experiments

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Experimental conditions used to perform western blot experiments

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Western Blot

Effect of prenatal VPA exposure on astrocytes in infancy. Analysis of the neurotrophin S100B and the GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) infant rats (PND 13). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses are normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfCGL and MLof Cb, and CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of prenatal VPA exposure on astrocytes in infancy. Analysis of the neurotrophin S100B and the GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) infant rats (PND 13). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses are normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfCGL and MLof Cb, and CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining

Effect of the prenatal VPA exposure on astrocytes in adolescence. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adolescent rats (PND 35). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; ** p < 0.01; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of the prenatal VPA exposure on astrocytes in adolescence. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adolescent rats (PND 35). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; ** p < 0.01; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining

Effect of the prenatal VPA exposure on astrocytes in adulthood. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adult rats (PND 90). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of the prenatal VPA exposure on astrocytes in adulthood. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adult rats (PND 90). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining

Experimental conditions for immunofluorescence.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease: In Vitro and In Vivo Evidence

doi: 10.1155/2018/4720532

Figure Lengend Snippet: Experimental conditions for immunofluorescence.

Article Snippet: Rabbit α -S100B , Novus Biologicals , 1 : 250 0.5% BSA in TBS/0.25% triton X-100 , TRITC conjugated goat anti-rabbit IgG (H+L) 1 : 200, 0.5% BSA in TBS/0.25% triton X-100 , Jackson ImmunoResearch.

Techniques: Immunofluorescence

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease: In Vitro and In Vivo Evidence

doi: 10.1155/2018/4720532

Figure Lengend Snippet: Primer sequences used for RT-PCR.

Techniques:

Experimental conditions for Western blot from 3×Tg-AD mice astrocytes and FC.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease: In Vitro and In Vivo Evidence

doi: 10.1155/2018/4720532

Figure Lengend Snippet: Experimental conditions for Western blot from 3×Tg-AD mice astrocytes and FC.

Techniques: Western Blot

Study of parameters related to reactive astrogliosis in 3×Tg-AD and non-Tg primary astrocytes. (a) Representative fluorescent photomicrographs of GFAP (green) and (b) signal quantification in both non-Tg (white bar) and 3×Tg-AD (black bar) primary astrocytes. (c) Representative fluorescent photomicrographs of S100B (red) and (d) signal quantification in both non-Tg (white bar) and 3×Tg-AD (black bar) primary astrocytes. Nuclei were stained with DAPI (blue). Scale bar is 50 μ m. Fluorescence analysis is expressed as Δ F / F 0 . (e) Representative bands and Western blot densitometric analysis of (f) GFAP, (g) S100B, (h) iNOS, and (i) COX-2. β -Actin was used as loading control. Results are expressed as percentage of the mean control value (non-Tg cells). Experiments were performed three times in triplicate. Data are presented as mean ± SEM. The statistical analysis was performed by Student's t -test ( ∗ P < 0.05 and ∗∗ P < 0.01 versus non-Tg group).

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease: In Vitro and In Vivo Evidence

doi: 10.1155/2018/4720532

Figure Lengend Snippet: Study of parameters related to reactive astrogliosis in 3×Tg-AD and non-Tg primary astrocytes. (a) Representative fluorescent photomicrographs of GFAP (green) and (b) signal quantification in both non-Tg (white bar) and 3×Tg-AD (black bar) primary astrocytes. (c) Representative fluorescent photomicrographs of S100B (red) and (d) signal quantification in both non-Tg (white bar) and 3×Tg-AD (black bar) primary astrocytes. Nuclei were stained with DAPI (blue). Scale bar is 50 μ m. Fluorescence analysis is expressed as Δ F / F 0 . (e) Representative bands and Western blot densitometric analysis of (f) GFAP, (g) S100B, (h) iNOS, and (i) COX-2. β -Actin was used as loading control. Results are expressed as percentage of the mean control value (non-Tg cells). Experiments were performed three times in triplicate. Data are presented as mean ± SEM. The statistical analysis was performed by Student's t -test ( ∗ P < 0.05 and ∗∗ P < 0.01 versus non-Tg group).

Techniques: Staining, Fluorescence, Western Blot, Control

Effect of chronic um-PEA on reactive astrogliosis and A β (1–42) expression in the FC of 3×Tg-AD and non-Tg mice. (a) Representative bands from RT-PCR performed in FC homogenates for GFAP, S100B, iNOS, and COX-2, and (b–e) densitometric analysis of the corresponding signals normalized to GAPDH. (f) Representative immunoreactive species and Western blot densitometric analysis of (g) GFAP, (h) S100B, (i) COX-2, (j) iNOS, and (k) A β (1–42) . β -Actin was used as loading control. Results are expressed as percentage of the mean control value (non-Tg/placebo). Experiments were performed three times in triplicate. Data are presented as mean ± SEM. The statistical analysis was performed by two-way ANOVA followed by Bonferroni's post hoc multiple comparison test ( ∗ P < 0.05 and ∗∗∗ P < 0.001 versus non-Tg/placebo group; ° P < 0.05, °° P < 0.01, and °°° P < 0.001 versus 3×Tg-AD/placebo group).

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease: In Vitro and In Vivo Evidence

doi: 10.1155/2018/4720532

Figure Lengend Snippet: Effect of chronic um-PEA on reactive astrogliosis and A β (1–42) expression in the FC of 3×Tg-AD and non-Tg mice. (a) Representative bands from RT-PCR performed in FC homogenates for GFAP, S100B, iNOS, and COX-2, and (b–e) densitometric analysis of the corresponding signals normalized to GAPDH. (f) Representative immunoreactive species and Western blot densitometric analysis of (g) GFAP, (h) S100B, (i) COX-2, (j) iNOS, and (k) A β (1–42) . β -Actin was used as loading control. Results are expressed as percentage of the mean control value (non-Tg/placebo). Experiments were performed three times in triplicate. Data are presented as mean ± SEM. The statistical analysis was performed by two-way ANOVA followed by Bonferroni's post hoc multiple comparison test ( ∗ P < 0.05 and ∗∗∗ P < 0.001 versus non-Tg/placebo group; ° P < 0.05, °° P < 0.01, and °°° P < 0.001 versus 3×Tg-AD/placebo group).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Comparison

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rabbit α-s100b (Agilent technologies)

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